Behavioral effects of dextran-induced intravascular aggregation of red blood cells (sludge) in the rabbit.

by Karl Michael Brooks

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  • Blood -- Agglutination.,
  • Behaviorism (Psychology).,
  • Rabbits.
The Physical Object
Paginationix, 171 leaves.
Number of Pages171
ID Numbers
Open LibraryOL23411510M

For red blood cells, the overall deformability (resistance to flow into ~3μm pipettes) can be tested, as well as more specific measurements of the contributing factors; cell geometry and membrane visco-elasticity. White blood cells and, rarely, platelets have also been subjected to . Absorbance values of the supernatant were , and from 1 mg/ml, 7 mg/ml and 10 mg/ml GNP-Dex treated red blood cells, for untreated cells and for Triton X treated cells. Effect of Dextran on Blood Typing and Crossmatching PAT ROCHE, JR., M.D., PAT ROCHE, JR., M.D. 1 Departments of Biochemistry and Medicine, Emory University School of Medicine, and the Medical Services of Lawson VA Hospital and Grady Memorial Hospital, . The Granulocyte Tragedy --Multicenter Trial of Hemodilution in Acute Ischemic Stroke --Similar and Opposite Hemorheological Effects of Dextran 40 and Hydroxyethyl Starch in Hemodilution Therapy for Stroke --Prognostic Importance of Blood Rheology in Acute Stroke --Hemispheric Cerebral Blood Flow Changes with Hypervolemic Hemodilution Using.

Plasma (% TBW) makes up the intravascular portion of extracellular fluid, and its fluid distribution will become extremely important when discussing fluid resuscitation. Water retained by the red and white blood cells is considered part of the intracellular fluid compartment because of their plasma membranes. Indeed, many investigators have concluded that all the beneficial effects of dextran can be ascribed to blood dilution 8,10–14, and evidence has appeared showing that this agent, like. A)cells engulf and digest microbes that want to attach to cell membranes and destroy normal cell function. B)produce cells that will be the first responder cells to protect against cancer formation. C)stimulate the person's immune system so that he or she can kill his or her own cancer cells. Rheology for All - Free download as Powerpoint Presentation .ppt /.pptx), PDF File .pdf), Text File .txt) or view presentation slides online. rheology of body fluids.

  Microvascular Rheology and Hemodynamics Microvascular Rheology and Hemodynamics LIPOWSKY, HERBERT H. INTRODUCTION At the birth of the Microcirculatory Society in , relatively little was known of the rheological behavior of blood in the microcirculation. The seminal studies of Poiseuille, Landis, Fåhraeus, Vejlens, and Krogh provided a conceptual . Many of the commonly used injectable corticosteroids have particulate compositions with molecular structures that are larger than the size of a red blood cell. 25 The increased particulate size creates the potential for distal capillary occlusion and resultant neurologic/spinal cord ischemia. Subsequent edema can trigger further ischemia. A mutant of wild-type Streptococcus sobrinus has been isolated which resists aggregation by exogenous dextran. This variant is able to form adherent plaque deposits in vitro when cultured in the presence of sucrose and has dextranase activity. In these respects it is the complement of previously described isolates which are plaque formation defective but aggregation normal. Dextran is a colloid effective for volume expansion; however, a possible side effect of its use is anaphylaxis. Dextran-induced anaphylactoid reaction (DIAR) is a rare but severe complication, with a small dose of dextran solution sufficient to induce anaphylaxis. An year-old female who underwent clipping for a ruptured cerebral aneurysm was admitted to the intensive care unit.

Behavioral effects of dextran-induced intravascular aggregation of red blood cells (sludge) in the rabbit. by Karl Michael Brooks Download PDF EPUB FB2

Behavioral effects of dextran-induced intravascular aggregation of red blood cells (sludge) in the rabbit Item menu. ACKNaYLEDGllENTS ThisworkwassupportsdbytheAgriculturalExpartmentStation andwascarriedoutinthelaboratoriesoftheDepartmentofFood Technology''andNutrition. Behavioral effects of dextran-induced intravascular aggregation of red blood cells (sludge) in the rabbit.

By Karl Michael Brooks. Abstract (Thesis) Thesis -- University of Florida, (Bibliography) Bibliography: leaves (Additional Physical Form) Also Author: Karl Michael Brooks.

The reversible aggregation of human red blood cells (RBC) by proteins or polymers continues to be of biologic and biophysical interest, yet Behavioral effects of dextran-induced intravascular aggregation of red blood cells book mechanistic details governing the process are still being explored.

Although a depletion model with osmotic attractive forces due to polymer depletion near the RBC surface has been proposed for aggregation by the neutral polyglucose dextran, its Cited by: the depletion model of RBC aggregation and is consistent with the predictions of the bridging mechanism.

Literature reports point out that elastic behavior of dextran molecules in low and high I regions is fairly typical of Hookean springs and hence predict an increase in tau(c) with increasing I.

A plateau step isCited by:   Here we present measurements on the dextran induced aggregation of red blood cells by use of atomic force microscopy based single cell force spectroscopy (SCFS).

The effects. A longer plateau step for suspensions with a higher molecular weight dextran is explained by a larger contribution of the enthalpic component to the dextran elasticity. Thus, the results reported in this study provide evidence that RBC aggregation is caused by the formation of dextran bridges between the cells.

Prior studies of dextran-induced RBC aggregation have focused on molecular mass ≤2 MDa, and thus a series of measurements were conducted using five molecular mass dextran fractions (i.e., 70,and kDa; 2 and 28 MDa) at concentrations of ≥2 g/ viscosities of the suspending phase solutions are shown in Fig.

aggregation results for 3–5 different donors are presented. The behavioral effects of dextran under these procedures were limited to a general, dose-dependent suppression of responding.

Finally, the incidence and extent of blood sludging in behaviorally impaired, aged Rhesus monkeys was compared with that of normal, young monkeys using ESR. The effects of ionic strength and cationic valency of the fluid medium on the surface potential and dextran-induced aggregation of red blood cells (RBC's) were investigated.

Dextran used for Blood Cell Separation Dextran can be applied in the reversible aggregation of human red blood cells, yet the mechanistic details governing the process are still being explored. The process is useful for separation of red blood cells from other cells and components of the blood.

The viscosity changes at high shear rates after dextran infusion would be due mainly to elevation of plasma viscosity, whereas the changes at low shear rates would be attributed to red blood cell aggregation induced by the dextran treatment.

This is a preview of subscription content, log in to check access. Red blood cell (RBC) aggregation and iron status are interrelated and strongly influenced by dietary factors, and their alterations pose a great risk of dyslipidemia and metabolic syndrome (MetS).

Currently, RBC aggregation-related dietary patterns remain unclear. This study investigated the dietary patterns that were associated with RBC aggregation and their predictive effects on.

HSC behavior in PV. (A) HSC physiology. Current concepts of the HSC hierarchy. 13 At the apex of the HSC hierarchy is the long-term HSC (LT-HSC; CD34 + CD38 − cell), which is responsible for lifetime maintenance of bone marrow cellular integrity and in which all MPN driver mutations are expressed.

In addition to the classical commitment pathway through the short-term HSC (ST-HSC), LT-HSCs. aggregation, the presenceofaggregates in turn influences the fluid flow. For example, at low rates of shear, blood flow obeys the Casson equation (Fung, ; Cokelet et al., ).

This non-Newtonian behavior is felt to reflect red cell aggregation, among other processes. There is to our knowledge no complete theory of aggregation and. Dextran 40 has been used to prevent post-operative thrombosis. However, its antithrombotic and antiplatelet effects in peripheral arterial disease (PAD) are poorly understood.

We studied the in vitro effects of Dextran 40 on platelet function in control subjects and PAD patients using whole blood methods. Platelet function was assessed in 20 control subjects and 20 PAD patients. Dextran increased the plasma viscosity and induced erythrocyte aggregation by forming dextran bridges between the cells [ 8, 9 ].

Hydroxyethyl starch (HES) / infusion elevated blood viscosity and erythrocyte aggregation while decreasing the erythrocyte deformability [ 6 ].

Gelatin was also found to increase erythrocyte aggregation [ 10 ]. Macro proteins like fibrinogen and globulins increase blood viscosity by interacting with RBCs and producing RBC aggregation.

30 Fibrinogen induced RBC aggregation is a very specific process due to RBC-to-RBC binding, where as PEs induced RBC aggregation includes multiple factors. 31 The mechanism for dextran-induced RBC aggregation is. Dextran is an α-D-1,6-glucose-linked glucan with side-chains linked to the backbone units of the Dextran biopolymer.

The degree of branching is approximately 5% in native (unfractionated) dextran and decreases with decreasing molecular weight. Intravascular coagulation is clearly visible in the blood vessels of the eye when viewed under relatively low level (60x) magnification.

Blood is in constant motion at constant temperature and the pH of blood is fixed at tobut its concentration of electrolytes is. Hemoglobin (American English) or haemoglobin (British English) (Greek αἷμα (haîma, “blood”) + -in) + -o- + globulin (from Latin globus (“ball, sphere”) + -in) (/ ˈ h iː m ə ˌ ɡ l oʊ b ɪ n, ˈ h ɛ-,-m oʊ-/), abbreviated Hb or Hgb, is the iron-containing oxygen-transport metalloprotein in the red blood cells (erythrocytes) of almost all vertebrates (the exception being the.

This is a good indication that a spiraling of our interests has taken place with the effects of the initial good work felt, not just in one corner of the globe, but in all four.

Intravascular Aggregation of Blood Cells and Tissue Metabolic Defects. Platelet Aggregates Induced by Red Blood Cell Injury. Pages Micromechanics of the Red Cell in Viscometric Flow. Pages Fischer, T. (et al.) Preview Buy Chap19 € Effect of extracorporeal circulation with different oxygenators on the volume of red blood cells.

Basic Aspects of Blood Trauma Book Subtitle. The authors have studied the effect of acute intracranial hypertension produced by placement of an epidural balloon (control group) in. The Hydrodynamic Radii of Macromolecules and Their Effect on Red Blood Cell Aggregation.

Biophysical Journal, 87, In article CrossRef PubMed [9] Pribush, A.; Zilberman-Kravits, D.; Meyerstein, N. The mechanism of the dextran-induced red blood cell Aggregation. European Biophysical Journal, 36, The higher-MW dextrans and HES cause an increase in plasma viscosity, and the larger dextrans (e.g., dextran 70) and gelatins also tend to cause red cell aggregation.

These effects are smaller in magnitude than the dilutional decrease in whole blood viscosity, but investigators are divided as to whether blood flow and tissue oxygenation can be. This allows for platelet aggregation and clot formation but preventing excessive accumulation of the clot, thus maintaining blood flow around the site of the clot.

Endothelial cells regenerate active cyclooxygenase faster than platelets because mature platelets cannot synthesize the enzyme, requiring new platelets to enter the circulation.

AIM To examine in vitro the effects of brief contact with various infusion solutions on red blood cells from newborn infants, as occurs in the “waste” syringe during routine blood sampling from umbilical artery catheters. The mixture of blood and solution in the “waste” syringe is usually reinfused into the baby.

Reinfused red blood cells may be damaged by the infusion solution. The Effects of Stroma-Free and Dextran-Conjugated Hemoglobin on Hemodynamics and Carotid Blood Flow in Hemorrhaged Guinea Pigs.

Artificial Cells, Blood Substitutes, and. Red cells elasticity increased from ± to ± mPa.s (Paggregation index rose from ± to ± (Pred cell aggregability may lower the velocity of erythrocyte transfer inside the tissue capillaries after HD, which may affect tissue perfusion.

Sherwood JM, Dusting J, Kaliviotis E, Balabani S. The effect of red blood cell aggregation on velocity and cell-depleted layer characteristics of blood in a bifurcating microchannel. Biomicrofluidics. (2). / [38] Kaliviotis E, Sherwood JM, Dusting J, Balabani S.Disseminated Intravascular Coagulation, also known as DIC, is a pathological activation of blood clotting mechanisms that may happen in response to a variety of diseases, or illnesses.

However, DIC, is most commonly observed in severe sepsis and septic shock.2. T. L. Fabry, “Mechanism of erythrocyte aggregation and sedimentation,” Blood 70(5), (). 3. A. V. Priezzhev, N. F. Nikolai, and J. Lademann, “Light backscattering diagnostics of red blood cell aggregation in whole blood samples,” Chapter 11 in Handbook of optical biomedical diagnostics, Tuchin V.